Composite

Part:BBa_K2200014

Designed by: Runqi Zhu   Group: iGEM17_Shenzhen_SFLS   (2017-10-31)


This part can specially cleavage mutant BRAF gene in melanoma cells with the regulation of miRNA



Usage and Biology

This part can specially cleavage mutant BRAF gene in melanoma cells with the regulation of miRNA. For more information please go to http://2017.igem.org/Team:Shenzhen_SFLS/Design


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3643
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 3649
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3768
    Illegal SapI.rc site found at 1159
    Illegal SapI.rc site found at 1401


Functional Parameters

[edit]
Categories
Parameters
None